In Vitro Assay of Staphylococcal Enterotoxins A and B from Milk
نویسندگان
چکیده
منابع مشابه
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per ...
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Pyrogenic responses, ranging up to 4.8 F, were induced in cats by oral administration of highly purified staphylococcal enterotoxin B in doses from 10 to 100 mug/kg. Fever was a more sensitive indicator of intoxication than was emesis. Highly purified preparations of enterotoxin A, whether administered intravenously (0.01 to 1.0 mug/kg), orally (10 to 25 mug/kg), or into the cerebral ventricles...
متن کاملStaphylococcal Enterotoxins
Staphylococcus aureus (S. aureus) is a Gram positive bacterium that is carried by about one third of the general population and is responsible for common and serious diseases. These diseases include food poisoning and toxic shock syndrome, which are caused by exotoxins produced by S. aureus. Of the more than 20 Staphylococcal enterotoxins, SEA and SEB are the best characterized and are also reg...
متن کاملThermal inactivation of staphylococcal enterotoxins B and C.
Thermal inactivation profiles of staphylococcal enterotoxins B (SEB) and C (SEC) at 80, 100, and 121 C showed that SEC is more resistant than SEB to heat. After 24 h of incubation at 25 C, some reactivation (recovery of serological reactivity) occurred in toxins that had been inactivated by heat. If the toxin was stirred during heating, reactivation did not occur. An examination of the reactiva...
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This study describes a multiplex PCR assay developed for the detection of Staphylococcus aureus enterotoxin types: SEA, SEB, SEC, SED, and SEE presented in the literature as classical. The method was then used to analyse the presence of genes encoding these enterotoxins in .S aureus strains isolated from raw milk. A total of 237 raw milk samples were used in the study and 77 (32.5%) of them wer...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1965
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(65)88245-5